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pH3H2
pH3H2
規(guī)格:
貨期:
編號:B240371
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pH3H2
商品貨號 B240371
Designations pH3H2
Species Homo sapiens, human
Vector
Construct size (kb): 5.599999904632568
Insert
DNA: genomic
Insert lengths(kb): 2.0
Gene product: macrophage stimulating 1 (hepatocyte growth factor-like)(hepatocyte growth factor-like) [D1S1]
Alleles: B2, B2, B2, B2, B1, B1, B1, B1, A2, A2, A1, A2, A1, A1
Insert Size (kb) 2.0
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Insert contains AvaI (2), PstI, KpnI (2), and BamHI sites.
Restriction digests of the clone give the following sizes (kb): EcoRI--5.6; PstI--3.0, 2.6; BamHI--3.9, 1.7; HindIII--3.5, 1.95; AvaI--2.7, 1.5, 1.2.
Detects loss of heterozygosity in renal cell carcinoma.
Derived from Ch4A-lambdaH3 (DNF15S2, formerly D1S1, p01970).
This probe detects a genomic HindIII fragment of 2 kb from chromosome 3 and genomic HindIII fragments of 3.8 and 8 kb from chromosome 1. The copy number of the fragments on chromosome 1 is 6-8 per haploid genome.
This probe detects a StuI RFLP mapping to 1p36 and a HindIII RFLP mapping to 3p21. Can detect loss of heterozygosity in lung cancers.
The StuI polymorphism shows autosomal dominant inheritance because of the repetitive nature of the locus. Band sizes are 3.8 kb, 3.6 kb, 2.0 kb, and 1.2 + 0.6 kb.
References

Leduc F, et al. Loss of heterozygosity in a gene coding for a thyroid hormone receptor in lung cancers. Am. J. Hum. Genet. 44: 282-287, 1989. PubMed: 2536219

Welch HM, et al. Transposition, amplification, and divergence in the origin of the DNF15 loci, a polymorphic repetitive sequence family on chromosome 1 and 3. Genomics 5: 423-430, 1989. PubMed: 2575580

Carritt B, et al. The D1S1 locus is part of a small dispersed sequence family (DNF15). Cytogenet. Cell Genet. 40: 559-600, 1985.

Zbar B, et al. Loss of alleles of loci on the short arm of chromosome 3 in renal cell carcinoma. Nature 327: 721-724, 1987. PubMed: 2885753

Kok K, et al. Deletion of a DNA sequence at the chromosomal region 3p21 in all major types of lung cancer. Nature 330: 578-581, 1987. PubMed: 2825033

Benjamin Carritt, personal communication

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