| 產(chǎn)品名稱 |
HRRM2 |
| 商品貨號(hào) |
B238376 |
| Designations |
HRRM2 |
| GenBank Number |
X59618
|
| Species |
Homo sapiens, human |
| Depositors |
Y Yen |
| Vector |
Construct size (kb): 5.125 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pCRII
Intact vector size: 3.900
Type of vector: phagemid
Vector end: TA site
Vector end: TA site
Cloning sites: TA cloning between EcoRI sites
Polylinker sites: NsiI HindIII KpnI SacI BamHI SpeI BstXI EcoRI EcoRI EcoRV
BstXI NotI AvaI XmaIII XhoI NsiI XbaI ApaI
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR, kanR
promoter: lac, SP6, T7
replicon: pMB1
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: RRM2
Gene name: ribonucleotide reductase M2 polypeptide
Contains complete coding sequence?: Y
Chromosome: 2; Localization: 2 p25-p24
Type of DNA: cDNA
Insert 5' end: Modification: BamHI site
Insert 3' end: Modification: HindIII site
Insert size (kb): 1.17
Cross references: DNA Seq. Acc.: X59618
Nucleotides ~1~1169 of the insert correspond to
nucleotides 195-1364 of X59618. Insert lengths(kb): 1.169999957084656 Gene product: ribonucleotide reductase (ribonucleoside-diphosphate reductase) small subunit( ribonucleotide reductase, small subunit, ribonucleotide reductase M2 polypeptide) [RRM2] |
| Insert Size (kb) |
1.170 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI--3.8, 1.2; HindIII--4.9; HindIII/EcoRI--3.9, 1.2; PstI--3.2, 1.1, 0.7; SalI--4.9. The insert contains the following restriction sites (approximate kb from the 5' end): SalI--0.08; PstI--0.68; EcoRI--1.14. Constructed by PCR using primers that generated a BamHI site upstream of the initiation codon (5' ATCCGGATCCACTATG 3') and a HindIII site downstream of the termination codon (5' TAAATAAGCTTAAGC 3'). |
| References |
Pavloff N, et al. Sequence analysis of the large and small subunits of human ribonucleotide reductase. DNA Seq. 2: 227-234, 1992. PubMed: 1627826
Yun Yen, personal communication
|
